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+WORD: This avoids the coupling of amino acids in the further cycles with the membrane as an alternative of the rising peptide chain. NOTE: After the coupling of the primary C-terminal amino acid of all peptides to the amino group of the membrane, the amino groups between the spots and in addition some of the amino teams throughout the spot areas do not form bonds with the amino acids. Ensure all the purification buffers are at 4 °C and carry out the protein purification within the cold room to keep away from protein denaturation.
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